primary human adult epidermal kcs Search Results


94
ATCC primary epidermal human kcs
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ATCC human adult primary epidermal keratinocytes kcs
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CELLnTEC Advanced Cell Systems AG human primary epidermal kcs (pooled)
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Lonza primary normal human epidermal keratinocytes
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Funakoshi ltd normal human epidermal kcs
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Kurabo industries human epidermal kcs
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Kurabo industries epidermal kcs
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Cell Applications Inc primary normal human epidermal kcs nheks
T-helper (Th)-1 cytokines promote SULT2B1 mRNA expression in human keratinocytes. <t>(A)</t> <t>Epidermal</t> CS levels in healthy controls (n = 30) and patients with the indicated skin diseases [seborrheic keratoses (n = 9), actinic keratoses (n = 10), tinea corporis (n = 3), atopic dermatitis (non-lesional, n = 9; lesional, n = 10), and psoriasis (non-lesional: n = 16; lesional: n = 37)] quantified via supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS; one-way ANOVA, followed by Dunnett’s multiple-comparison test). (B, C) Real-time PCR analysis of SULT2B1 gene levels in undifferentiated (B) or differentiated (C) normal human epidermal keratinocytes <t>(NHEKs)</t> treated with two concentrations of the indicated cytokines (n = 6/group; one-way ANOVA, followed by Dunnett’s multiple-comparison test). Data were obtained from six (B, C) independent experiments, and graphs are represented as the mean ± SD. ** P < 0.01 and *** P < 0.001; ns, not significant.
Primary Normal Human Epidermal Kcs Nheks, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Applications Inc normal neonatal human epidermal kcs
T-helper (Th)-1 cytokines promote SULT2B1 mRNA expression in human keratinocytes. <t>(A)</t> <t>Epidermal</t> CS levels in healthy controls (n = 30) and patients with the indicated skin diseases [seborrheic keratoses (n = 9), actinic keratoses (n = 10), tinea corporis (n = 3), atopic dermatitis (non-lesional, n = 9; lesional, n = 10), and psoriasis (non-lesional: n = 16; lesional: n = 37)] quantified via supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS; one-way ANOVA, followed by Dunnett’s multiple-comparison test). (B, C) Real-time PCR analysis of SULT2B1 gene levels in undifferentiated (B) or differentiated (C) normal human epidermal keratinocytes <t>(NHEKs)</t> treated with two concentrations of the indicated cytokines (n = 6/group; one-way ANOVA, followed by Dunnett’s multiple-comparison test). Data were obtained from six (B, C) independent experiments, and graphs are represented as the mean ± SD. ** P < 0.01 and *** P < 0.001; ns, not significant.
Normal Neonatal Human Epidermal Kcs, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


T-helper (Th)-1 cytokines promote SULT2B1 mRNA expression in human keratinocytes. (A) Epidermal CS levels in healthy controls (n = 30) and patients with the indicated skin diseases [seborrheic keratoses (n = 9), actinic keratoses (n = 10), tinea corporis (n = 3), atopic dermatitis (non-lesional, n = 9; lesional, n = 10), and psoriasis (non-lesional: n = 16; lesional: n = 37)] quantified via supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS; one-way ANOVA, followed by Dunnett’s multiple-comparison test). (B, C) Real-time PCR analysis of SULT2B1 gene levels in undifferentiated (B) or differentiated (C) normal human epidermal keratinocytes (NHEKs) treated with two concentrations of the indicated cytokines (n = 6/group; one-way ANOVA, followed by Dunnett’s multiple-comparison test). Data were obtained from six (B, C) independent experiments, and graphs are represented as the mean ± SD. ** P < 0.01 and *** P < 0.001; ns, not significant.

Journal: Frontiers in Immunology

Article Title: Sulfotransferase SULT2B1 contributes to the epithelial–immune microenvironment homeostasis in imiquimod-induced psoriatic dermatitis

doi: 10.3389/fimmu.2025.1632426

Figure Lengend Snippet: T-helper (Th)-1 cytokines promote SULT2B1 mRNA expression in human keratinocytes. (A) Epidermal CS levels in healthy controls (n = 30) and patients with the indicated skin diseases [seborrheic keratoses (n = 9), actinic keratoses (n = 10), tinea corporis (n = 3), atopic dermatitis (non-lesional, n = 9; lesional, n = 10), and psoriasis (non-lesional: n = 16; lesional: n = 37)] quantified via supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS; one-way ANOVA, followed by Dunnett’s multiple-comparison test). (B, C) Real-time PCR analysis of SULT2B1 gene levels in undifferentiated (B) or differentiated (C) normal human epidermal keratinocytes (NHEKs) treated with two concentrations of the indicated cytokines (n = 6/group; one-way ANOVA, followed by Dunnett’s multiple-comparison test). Data were obtained from six (B, C) independent experiments, and graphs are represented as the mean ± SD. ** P < 0.01 and *** P < 0.001; ns, not significant.

Article Snippet: Primary normal human epidermal KCs (NHEKs) isolated from adult skin (#CA10205a; Cell Applications, San Diego, CA, USA) were cultured in T-75 flasks with the Human EpiVita Serum-Free Growth Medium Kit for Adult Cells (#A141K500a; Cell Applications) containing 0.06 mM Ca 2+ , according to the manufacturer’s instructions.

Techniques: Expressing, Supercritical Fluid Chromatography, Mass Spectrometry, Tandem Mass Spectroscopy, Comparison, Real-time Polymerase Chain Reaction